为什么双酶切有目的片段测序却没有...

Some of nucleotides in the sequence track CGTTTAAACT TAAGCTTG couldbe disappeared, but AGCTT should be remained in the clone, or how didyou cut off the insert, excepted that there is another Hind III sitein the insert. You need to upload your gel pictures with those enzyme digestions.

左面的4个是酶切后的“重组质粒”，质粒大小3000左右，目的片段大概1670左右，最左面是2000的dna marker

我觉得是因为有些片段出现质粒断裂导致的结果 你可以多挑几个去测序试试

帆帆0816：送了几次测序了，都是这个结果，丢失了这部分碱基，是质粒断裂导致的吗

Do you have DNA sequencing results that show sequences from bottom strand?

Could you label the gel picture? Do you have any single enzymedigestion gel picture?