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超氧歧化酶检测试剂盒
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氯磷酸二钠脂质体 Clo
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SCILOGEX赛洛捷克 MS-| 货号： | ALX-350-268-MC01 |
| 保存条件： | -20 |
| 供应商： | 上海伟进生物科技有限公司 |
| 英文名： | Phalloidin (FITC) |
| 保质期： | 长期 |
For detection of microfilaments in the light microscope. Mixture of 4 isomers with lower affinity to actin than phalloidin (Prod. No. ALX-350-265).
Product Specification
| Alternative Name: | Fluoresceinyl-aminomethyldithiolano-phalloidin |
| Formula: | C58H62N10O14S4 |
| MW: | 1251.4 |
| Source: | Semisynthetic from aminomethyldithiolano-phalloidin. |
| Purity: | ≥90% (HPLC) |
| Appearance: | Amorphous solid. |
| Solubility: | Soluble in DMSO, dimethyl formamide, methanol or water (warm). |
| Long Term Storage: | -20°C |
| Use/Stability: | Prepare fresh solutions for each application. |
| Handling: | Protect from light and moisture. |
| Protocol: | Simultaneous Fixation, Permeabilization, and Fluorescent Phallotoxin Staining The phallotoxins appear to be stable for short periods in 4% formaldehyde fixation buffers. This permits a rapid one-step fixation, permeabilization, and labeling procedure as follows. Preparation Instructions Solutions should be prepared fresh and protected from light when ever possible. Solubility in water (0 °C): 0.5%; much more soluble in hot water; freely soluble in methanol, ethanol, butanol, and pyridine. Solubility of product in methanol at the following concentration: Phalloidin-FITC: 0.5 mg/ml Procedure Stock solutions of phalloidin conjugates have been made in methanol or DMSO at 0.1–5 mg/ml. Final staining solutions in aqueous physiological buffers are in the concentration range of 0.1–100 µM with corresponding incubation times of 15 minutes to 72hours. The following procedure may be used as a guideline for staining cells:Waggoner, A. et al., Methods in Cell Biology, 30, 449 (1989)
Emission: 513 nm Living Cells Phallotoxins are usually not cell-permeant and have therefore not been used extensively with living cells. However, living cells have been labeled. Pinocytosis appears to be the method of entry for some cells, although hepatocytes “avidly” take up the dye by an unknown mechanism. In general, a larger amount of stain will be needed for staining living cells. |
| 产品货号 | 产品名称 品牌 | 价格 |
|---|---|---|
| PHDR1 | Rhodamine Phalloidin 罗丹明标记 | 2163.00 |
| PHDH1-A | Acti-stain™ 555 phalloidin 红色荧光标记 现货 | 2451.00 |
| PHDN1-A | Acti-stain™ 670 phalloidin蓝色荧光标记 | 2451.00 |
| PHDG1-A | Acti-stain™ 488 phalloidin 绿色荧光标记 | 2451.00 |
| ALX-350-267-MC01 | Phalloidin (Biotin) | 3605.00 |
温馨提示：不可用于临床治疗。
