Fibrinogen, Biotin conjugated

product information

background

Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N-terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/ml. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel et al. 2000;  Rapold et al. 1989).

immunogen

purified, full length native fibrinogen Q9UE34

antibody format

hen;

polyclonal;

affinity purified IgY at 1mg/ml in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1 % sodium azide, pH 7.2;

liquid

quantity

200 µl

storage

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

tested applications

Western blot (WB), immunodifusion (ID)

related products

IMS09-038-335 Fibrinogen | labelled with fluorescien
 

additional information

to be added when available

application information

recommended dilution

1: 5000 (WB)

expected | apparent MW

75 kDa

confirmed reactivity

human

predicted reactivity

bovine, mouse, porcine, rabbit, rat

not reactive in

no confirmed exceptions from predicted reactivity known in the moment

additional information

only one precipitation arc in immunodiffusion and immunoelectrophoresis against normal human plasma or human fibrinogen

selected references

to be added when available

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