Anti-Acetylcholinesterase (human brain, bovine brain, AChE)

Cat. No.

HYB 190-015

Product name

Anti-Acetylcholinesterase (human brain, bovine brain, AChE)

Description

Mouse monoclonal antibody

Specificity

HYB 190-01 is specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and erythrocyte AChE. 

Immunogen

C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the erythrocyte enzyme. 

Application

ELISA, WB, IHC

Species reactivity (positive)

Human, bovine, Torpedo marmorata

Species reactivity (negative)

Electric eel, human

Subclass

IgG1/l

Gene ID

43, 540446

Clone number

4E11

Epitope

C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE) (1).

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa.
Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.
Application 1
ELISA
Can be used in ELISA on amniotic fluid for the diagnosis of neural tube defects.  
HYB 190-01 is well suited as catching antibody (on an anti-mouse IgG coat) in enzyme antigen immunoassay (EAIA), where the antigen (AChE) is captured and used directly as substrate for acetylthiocholiniodide (Ellmann’s reaction) (1).
Application 2
WB
In Western blotting and dot blotting HYB 190-01 reacts with native and denatured human and bovine, detergent soluble and salt-soluble AChE. No cross-reactivity is seen with erythrocyte AChE..In Western blotting a dilution guideline of 1/75 has proved successful (1). 
Application 3
IHC
HYB 190-01 can be uesd in Immunohistochemistry.
Human placenta stained with HYB 190-01