Cynomolgus LDL R Protein

货号：	LDR-C5222
供应商：	ACROBiosystems
数量：	In Stock
英文名：	Cynomolgus LDL R Protein
规格：	100ug/1mg

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus LDL R, His Tag (Catalog # LDR-C5222) at 2μg/mL (100 μL/well) can bind Biotinylated Human PCSK9, His Tag (Catalog # PC9-H82E7) with a linear range of 0.007-0.256 μg/mL.

产品背景:
Low-Density Lipoprotein (LDL) Receptor is also known  as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.

产品描述:
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 86.8 kDa. The protein migrates as 135-155 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
 

SDS-PAGE

Cynomolgus LDL R, His Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.