Human Interleukin 6 (IL-6) ELISA Kit

货号：	AE62763HU
样本：	Serum, Plasma, Other biological fluids
标记物：	P05231
适应物种：	Human (Homo sapiens)
应用：	Quantitative
检测方法：	Sandwich
检测限：	3.2 pg/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：IL6
别名：BSF2; HGF; HSF; IFNB2; IL-6; B cell stimulatory factor-2|B-cell differentiation factor|CTL differentiation factor|OTTHUMP00000158544|hybridoma growth factor|interleukin 6|interleukin BSF-2
检测范围：7.8-500 pg/mL
产品概述：IL-6 is a multifunctional protein produced by lymphoid and non-lymphoid cells, and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these various cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. On the basis of its various activities, IL-6 has also been called interferon-β2 (IFN-β2), 26 kDa protein, B-cell stimulatory factor-2 (BSF-2),　hybridoma/plasmacytoma growth factor, hepatocyte stimulating factor, cytotoxic T-cell differentiation factor, and macrophage-granulocyte inducing factor 2A (MGI-2A).
特异性：This assay has high sensitivity and excellent specificity for detection of Human IL6. No significant cross-reactivity or interference between Human IL6 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Human IL6 and the recovery rates were calculated by comparing the measured value to the expected amount of Human IL6 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human IL6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate IL6 in samples. An antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.