Mouse Mucin-2 (MUC2) ELISA Kit

货号：	AE58288MO
样本：	Serum, Plasma, Other biological fluids
标记物：	Q80Z19
适应物种：	Mouse (Mus musculus)
应用：	Quantitative
检测方法：	Sandwich
检测限：	0.30 ng/mL
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：MUC2
别名：MLP; SMUC; mucin 2|mucin 2; intestinal/tracheal|mucin-like protein
检测范围：0.78-50 ng/mL
产品概述：Mucin 2 is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lining into the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins, polymerizes into a gel of which 80% by weight is oligosaccharide side chains which are added as post-translational modifications to the mucin proteins. This gel provides an insoluble mucous barrier that serves to protect the intestinal epithelium.The mucin 2 protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
特异性：This assay has high sensitivity and excellent specificity for detection of Mouse MUC2. No significant cross-reactivity or interference between Mouse MUC2 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Mouse MUC2 and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse MUC2 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse MUC2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate MUC2 in samples. An antibody specific for MUC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.