Pig Peroxiredoxin-6 (PRDX6) ELISA Kit

货号：	AE26072PI
样本：	Serum, Plasma, Other biological fluids
标记物：	Q9TSX9
适应物种：	Pig (Sus scrofa; Porcine)
应用：	Quantitative
检测方法：	Sandwich
检测限：	Request Information
供应商：	华夏远洋
数量：	999
规格：	96T/48T

简写：PRDX6
别名：1-Cys; AOP2; KIAA0106; MGC46173; NSGPx; PRX; aiPLA2; p29; 1-Cys peroxiredoxin|acidic calcium-independent phospholipase A2|antioxidant protein 2|non-selenium glutathione peroxidase
检测范围：Request Information
产品概述：Peroxiredoxin-6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.Kim et al. (1997) isolated a calcium-independent lysosomal PLA2 from a human myeloblast cell line. The predicted 224-amino acid protein contained the PLA2 catalytic motif, but no other significant homology to known phospholipases. Expressed protein had significant PLA2 activity on several substrates.
特异性：This assay has high sensitivity and excellent specificity for detection of Pig PRDX6. No significant cross-reactivity or interference between Pig PRDX6 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Pig PRDX6 and the recovery rates were calculated by comparing the measured value to the expected amount of Pig PRDX6 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pig PRDX6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate PRDX6 in samples. An antibody specific for PRDX6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDX6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX6 bound in the initial step. The color development is stopped and the intensity of the color is measured.