货号：	ACP-005
抗体名：	Anti-PMCA1
抗体英文名：	Anti-PMCA1
靶点：	见官方网站
浓度：	见官方网站
应用范围：	IH, WB
宿主：	Rabbit
供应商：	上海信裕生物科技有限公司
数量：	大量
级别：	高
目录编号：	ACP-005
抗原来源：	Rabbit
保质期：	6个月
适应物种：	见官方网站
标记物：	见官方网站
克隆性：	否
保存条件：	-20°C
形态：	液体或冻干粉
亚型：	见官方网站
免疫原：	见官方网站
规格：	25 µl, 50 µl, 0.2 ml

Anti-PMCA1

Plasma membrane Ca²⁺ ATPase 1, ATP2B1
Cat #: ACP-005
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
May also work in: IC, IFC, IP
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
CLICK HERE TO RECEIVE A 25 µl FREE TRIAL SAMPLE!

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human PMCA1. Anti-PMCA1 antibody (#ACP-005) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA1 from mouse, rat, and human samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:
1, 2. Anti-PMCA1 antibody (#ACP-005), (1:200).
3, 4. Anti-PMCA1 antibody, preincubated with the control peptide antigen.
Immunohistochemistry

Expression of PMCA1 in mouse hippocampus
Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PMCA1 antibody (#ACP-005), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. PMCA1 staining (green) appears in the stratum radiatum (SR) and oriens (OR) layers but not in the pyramidal (Pyr) layer of hippocampal CA1 region (arrows). Nuclei are labeled with DAPI (blue).
Immunogen
Peptide (C)SGVKNSLKEANHD, corresponding to amino acid residues 12-24 of human PMCA1 (Accession P20020). Intracellular, N-terminus.

HomologyRat, mouse - identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
SpecificityThis antibody will recognize all known PMCA1 splice variants.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

The plasma membrane Ca²⁺ ATPase (PMCA) is a transport protein in the plasma membrane of cells responsible for removing calcium (Ca²⁺) from the cell. This pump is vital for regulating the amount of Ca²⁺ within cells¹.

PMCA and the Na⁺ calcium exchanger (NCX) are together the main regulators of intracellular Ca²⁺ concentrations².

Since it transports Ca²⁺ into the extracellular space, PMCA is also an important regulator of the Ca²⁺ concentration in the extracellular space³.

PMCA belongs to a family of P-type primary ion transport ATPases, and is expressed in a variety of tissues, including the brain⁴.

The pump is powered by the hydrolysis of adenosine triphosphate (ATP), with a stoichiometry of one Ca²⁺ ion removed for each molecule of ATP hydrolyzed. It binds to Ca²⁺ ions with a high affinity (a Km of 100 to 200 nM) but does not remove Ca²⁺ at a very fast rate. This is in contrast to NCX, which has a low affinity and a high capacity. Thus, PMCA is effective at binding Ca²⁺ even when its concentration within the cell is very low, so it is suited for maintaining Ca²⁺ at its normally very low levels. The NCX is better suited for removing large amounts of Ca²⁺ quickly, as it is needed in neurons after an action potential. Thus the activities of the two types of pump complement each other⁵.

References

1. Jensen, T.P. et al. (2004) Brain Res. Dev. Brain Res. 152, 129.
2. Strehler, E.E. and Zacharias, D.A. (2001) Physiol. Rev. 81, 21.
3. Talarico, E.F. et al. (2005) Mol. Vis. 11, 169.
4. Jensen, T.P. et al. (2007) J. Physiol. 579, 85.
5. Burette, A. and Weinberg, R.J. (2007) J. Comp. Neurol. 500, 1127.