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β-Catenin(β-连接素) 斑马鱼抗体
AADAT Polyclonal Antibody
Calretinin Antibody（钙结合蛋白抗体
[VIP第1年] 指数:3
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IV型胶原蛋白/4型胶原
MicroVue CICP EIA*| 货号： | STP-100-B |
| 抗体名： | ProTx-II-Biotin |
| 抗体英文名： | ProTx-II-Biotin |
| 靶点： | 见官方网站 |
| 浓度： | 见官方网站 |
| 应用范围： | 见官方网站 |
| 宿主： | Synthetic |
| 供应商： | 上海信裕生物科技有限公司 |
| 数量： | 大量 |
| 级别： | 高 |
| 目录编号： | STP-100-B |
| 抗原来源： | Synthetic |
| 保质期： | 6个月 |
| 适应物种： | 见官方网站 |
| 标记物： | 见官方网站 |
| 克隆性： | 否 |
| 保存条件： | -20°C |
| 形态： | 液体或冻干粉 |
| 亚型： | 见官方网站 |
| 免疫原： | 见官方网站 |
| 规格： | 0.1 mg, 5 x 0.1 mg, 10 x 0.1 mg |
ProTx-II-Biotin
β/ω-theraphotoxin-Tp2a-Biotin, Protoxin-2-Biotin, PT-II-Biotin, ProTx2-Biotin, Protoxin2-BiotinA Blocker of Nav1.7 and Nav1.5 Channels and some T-Type Ca2+ Channels Conjugated to Biotin
Cat #: STP-100-B
Sizes: 0.1 mg, 5 x 0.1 mg, 10 x 0.1 mg
Source: Synthetic
MW: 4053 Da.
Net Peptide Content: 100%
Target: NaV Na+ channels and T-type Ca2+ channels
ProTx-II is a peptidyl toxin originally produced in Thrixopelma pruriens, the Peruvian green velvet tarantula. It was identified as a voltage-gated Na+ channel (NaV) blocker which inhibits both tetrodotoxin-sensitive and tetrodotoxin-resistant channels1.
Binding of ProTx-II to an extracelluar domain of NaV channel, probably to a hydrophobic site3, inhibits current by shifting the channel activation to more positive potentials1-2.
ProTx-II Inhibits NaV1.2, NaV1.3, NaV1.5, NaV1.6, NaV1.7 and, NaV1.8. It is a significantly more potent inhibitor against NaV1.7 than the other NaV channel subtypes1.
ProTx-II was also found as an effective modulator which shifts the voltage dependence activity of T-type Ca2+channel1.
Alomone Labs is pleased to offer ProTx-II-Biotin (#STP-100-B), a synthetic peptide. The extent of labeling is 1 molecule of biotin per molecule of ProTx-II.
ProTx-II-BiotinReferences
- Middleton, R.E. et al. (2002) Biochemistry 41, 14734.
- Smith, J.J. et al. (2005) J. Biol. Chem. 280, 11127.
- Smith, J.J. et al. (2007) J. Biol. Chem. 282, 12687.
- Our Bioassay
- Specifications
- Related Products
Alomone Labs ProTx-II-Biotin inhibits NaV1.7 channels expressed in Xenopus oocytes.
A. Time course of ProTx-II-Biotin (#STP-100-B) inhibition of NaV1.7 channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage step to 0 mV every 10 sec, and was significantly inhibited by 200 nM ProTx-II-Biotin (bar), applied for 4.5 min. B. Superimposed traces of NaV1.7 current upon application of control and of 200 nM ProTx-II-Biotin (as indicated), taken from the recording shown in A.
Alomone Labs ProTx-II-Biotin specifically binds avidin.
SDS-PAGE resolved purified ProTx-II-Biotin (#STP-100-B) specifically binds peroxidase-conjugated avidin, showing a dose-dependent ECL signal at ~4 kDa, absent for the same amount of unlabeled ProTx-II (#STP-100).
OriginThrixopelma pruriens (Peruvian green velvet tarantula).
Purity>98%.
FormulationLyophilized Powder.
Effective concentration10-500 nM.
SequenceBiotin-YCQKWMWTCDSERKCCEGMVCRLWCKKKLW.
ModificationsDisulfide bonds between Cys2-Cys16, Cys9-Cys21, and Cys15-Cys25.
LabelBiotin at the N-terminus.
CAS numberNot determined.
ActivityProTx-II inhibits NaV channels1. ProTx-II could also modulate T-type Ca2+ channels at higher concentrations2.
Storage before reconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
ReconstitutionAny aqueous buffer. Centrifuge all product preparations before use (1000 X g 5 min).
Storage after reconstitutionUp to one week at 4°C or three months at -20°C.
