货号: | 220502 |
供应商: | 上海冠东生物科技有限公司 |
数量: | 大量 |
英文名: | α 2 Antiplasmin chromogenic assay |
保质期: | 1年 |
保存条件: | 2-8℃ |
规格: | 3x3ml |
BIOPHEN® α2-Antiplasmin (LRT) kit is a chromogenic assay for the quantitative determination of α2-Antiplasmin (α2-AP) activity in human citrated plasma using a manual or automated method. Reagents are in the liquid presentation, ready to use (LRT = Liquid reagent Technology).
SUMMARY AND EXPLANATION:
Alpha 2-Antiplasmin (or α2-Antiplasmin or plasmin inhibitor) is a serine protease inhibitor (serpin) responsible for inactivating plasmin, an important enzyme that participates in fibrinolysis and degradation of various other proteins. Assaying α2-AP activity may be useful information in case of α2-AP deficiency or during fibrinolytic therapy.
ASSAY PRINCIPLE:
α2-Antiplasmin present in the plasma sample inactivates plasmin (R1). The residual plasmin cleaves the specific substrate SPm-41 (R2), releasing paranitroaniline (pNA), which color is measured at 405nm. There is an inverse relationship between color development and α2-AP activity in the tested plasma.