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异氟烷，动物麻醉用异氟烷
一步法RNA提取试剂盒
MARK/Par-1 Activity Inhibitor, 3962
[VIP第1年] 指数:2
通过宝山区市场监督管理局认证 
革兰氏染色液试剂盒
巴氏染色液试剂盒
瑞氏染色液试剂盒
TianFect 转染试剂
Caspase 6 活性检测试
磷酸钙法细胞转染试剂
大鼠雪旺细胞| 货号： | DMSSY510 |
| 供应商： | 上海笃玛生物科技有限公司 |
| 数量： | 现货 |
| 保存条件： | 电询 |
| 规格： | 100T |
自噬MDC染色步骤
Labeling of Autophagic Vacuoles with monodansylcadaverine (MDC)The autofluorescent agent MDC (Sigma) was recently introduced as a specific autophagolysosome marker to analyze the autophagic process [33]. U251 cells were treated with Res 150 μM for 3–24 h. Autophagic vacuoles were labeled with MDC by incubating cells with 0.05 mM MDC in RPMI1640 at 37°C for 10 min at various times after Res treatment. After incubation, cells were washed three times with PBS and immediately analyzed with a fluorescence microscope (Nikon Eclipse TE 300, Japan) equipped with a filter system (V-2A excitation filter:380/420 nm, barrier filter:450 nm) [34]. Images were captured with a CCD camera and imported into Adobe Photoshop.
Labeling of Autophagic Vacuoles with monodansylcadaverine (MDC)The autofluorescent agent MDC (Sigma) was recently introduced as a specific autophagolysosome marker to analyze the autophagic process [33]. U251 cells were treated with Res 150 μM for 3–24 h. Autophagic vacuoles were labeled with MDC by incubating cells with 0.05 mM MDC in RPMI1640 at 37°C for 10 min at various times after Res treatment. After incubation, cells were washed three times with PBS and immediately analyzed with a fluorescence microscope (Nikon Eclipse TE 300, Japan) equipped with a filter system (V-2A excitation filter:380/420 nm, barrier filter:450 nm) [34]. Images were captured with a CCD camera and imported into Adobe Photoshop.
温馨提示：不可用于临床治疗。
