Anti-D-dimer (human, dog)

Cat. No.

ABS 015-225

Product name

Anti-D-dimer (human, dog)

Description

Mouse monoclonal antibody

Specificity

ABS 015-22 binds human D-dimer. Cross-reacts < 2% with directly coated D-monomer but not with directly coated fibrinogen.
 

Immunogen

Purified human D-dimer 

Application

ELISA, WB

Species reactivity (positive)

Human, dog

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

2243, 475473

Clone number

22

Epitope

When tested in a pair with itself, with ABS 015-28 and with a fibrinogen-specific polyclonal antibody, the epitope appears to relate to the dimenzation site of D-dimer.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

D-dimer is a fibrin degradation product, a small protein fragment present in the blood after a blood clot is degraded by fibrinolysis.

Application 1

ELISA
ABS 015-22 binds free D-dimer in solution. 
ABS 015-022 works as capture antibody in sandwich ELISA with biotinylated ABS 015-22 or ABS 015-28 as detection antibody.
Biotinylated ABS 015-22 works as detection antibody in sandwich ELISA for D-dimer with itself (ABS 015-22) or ABS 015-28 as capture antibody. 
The combination with ABS 015-28 as capture antibody and ABS 015-22B as detection antibody is recommended.
 

The calibration curve of a sandwich assay for D-Dimer using ABS 015-28 as capture antibody and ABS 015-22B as the biotinylated detection antibody.

Application 2

WB
Due to reducing conditions in Western blotting ABS 015-22 binds the alpha-chain (12kDa) of D-Dimer, and not the entire D-Dimer complex.