pPZP211载体

LOCUS Exported 9015 bp ds-DNA circular SYN 28-JUL-2016DEFINITION Agrobacterium binary vector for plant transformation, with spectinomycin- and kanamycin-resistance genes. The MCS is reversed in pPZP212.ACCESSION U10490VERSION .KEYWORDS pPZP211SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 9015) AUTHORS Hajdukiewicz P, Svab Z, Maliga P. TITLE The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. JOURNAL Plant Mol. Biol. 1994;25:989-94. PUBMED 7919218REFERENCE 2 (bases 1 to 9015) AUTHORS . TITLE Direct SubmissionCOMMENT The GenBank sequence was corrected by inserting a G at position 2249.FEATURES Location/Qualifiers source 1..9015 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" CDS 1187..1816 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /note="pVS1 StaA" /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 2245..3318 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /note="pVS1 RepA" /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 3384..3578 /note="pVS1 oriV" /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 3922..4062 /note="bom" /note="basis of mobility region from pBR322" rep_origin complement(4248..4836) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5080..5871) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase" /note="SmR" /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" misc_feature 6399..6423 /note="LB T-DNA repeat" /note="left border repeat from nopaline C58 T-DNA" polyA_signal 6521..6695 /note="CaMV poly(A) signal" /note="cauliflower mosaic virus polyadenylation signal" CDS complement(6749..7546) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(7606..8284) /note="35S promoter (enhanced)" /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" promoter 8511..8541 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 8549..8565 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8573..8589 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 8585..8818 /codon_start=1 /gene="lacZ (fragment)" /product="LacZ-alpha fragment of beta-galactosidase" /note="lacZ-alpha" /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC" misc_feature 8585..8587 /note="ATG" misc_feature 8599..8655 /note="MCS" /note="pUC18 multiple cloning site" primer_bind complement(8659..8675) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" misc_feature 8878..8902 /note="RB T-DNA repeat" /note="right border repeat from nopaline C58 T-DNA"ORIGIN 1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag 61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg 121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac 181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc 241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc 301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc 361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg 421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc 481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg 541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc 601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg 661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg 721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa 781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc 841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg 901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg 961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg 1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc 1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg 1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc 1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc 1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg 1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac 1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc 1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt 1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg 1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg 1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga 1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct 1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa 1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta 1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac 1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag 1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag 2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg 2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg 2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg 2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc 2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg 2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc 2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag 2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt 2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag 2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg 2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg 2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg 2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa 2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac 2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag 2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag 3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat 3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc 3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc 3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag 3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa 3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa 3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac 3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac 3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa 3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc 3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg 3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta 3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc 3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata 4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc 4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aatcgcttga gttaacgccg 5041 gcgaagcggc gtcggcttga acgaatttct agctagacat tatttgccga ctaccttggt 5101 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg 5161 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc 5221 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac 5281 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc 5341 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg 5401 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc 5461 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag 5521 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg 5581 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc 5641 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag 5701 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac 5761 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc 5821 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccccca tgatgtttaa 5881 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca 5941 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa 6001 aaaacatgtc ataacaagaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt 6061 cggtcaaggt tctggaccag ttgcgtgacg gcagttacgc tacttgcatt acagcttacg 6121 aaccgaacga ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct 6181 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc 6241 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca 6301 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt 6361 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta 6421 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt 6481 aacgccgaat taattcgggg gatctatagg gactttaggt gatctggatt ttagtactgg 6541 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat 6601 actaagggtt tcttatatgc tcaacacgtg agcgaaaccc tataagaacc ctaattccct 6661 tatctgggaa ctactcacac attattatgg agaaactcga tgtcgatcga ctctagctag 6721 aggatcgatc cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg 6781 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt 6841 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg 6901 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat 6961 tcggcaagca ggcatcgcca tgtgtcacga cgagatcctc gccgtcgggc atgcgcgcct 7021 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct 7081 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt 7141 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga 7201 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc 7261 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa 7321 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctggagttca ttcagggcac 7381 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg 7441 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc 7501 aagcggccgg agaacctgcg tgcaatccat cttgttcaat ccccatggtc gatcgacaga 7561 tctgcgaatc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc 7621 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc 7681 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg 7741 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc 7801 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc 7861 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag 7921 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac 7981 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca 8041 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg 8101 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt 8161 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg 8221 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt 8281 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt 8341 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg 8401 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 8461 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 8521 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 8581 agctatgacc atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg 8641 caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 8701 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 8761 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag 8821 cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga 8881 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt 8941 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt 9001 tcccctcggg atcaa//