Anti-Fibronectin (bovine, human)

Cat. No.

CSI 005-32

Product name

Anti-Fibronectin (bovine, human)

Description

Mouse monoclonal antibody

Specificity

CSI 005-32 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain. 

Immunogen

Bovine corneal endothelial cells

Application

ELISA, WB, IHC-F, IHC-P, IP

Species reactivity (positive)

Bovine, human

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

280794 (2335)

Clone number

A32

Epitope

Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of CSI 005-35

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities (1).