Anti-Mycobacterium tuberculosis ESAT-6

Cat. No.

HYB 076-08

Product name

Anti-Mycobacterium tuberculosis ESAT-6

Description

Mouse monoclonal antibody

Specificity

HYB 076-08 reacts with ESAT-6 in immunoblotting after SDS-PAGE of short-term culture filtrates of Mycobacterium tuberculosis and virulent strains of M. bovis. HYB 076-08 cross-reacts with MPB64 (26 kDa) from certain BCG substrains, with the A, B, and C components of the antigen 85 complex and with MPT51. H

Immunogen

Purified protein derivative (PPD) of tuberculin from M. tuberculosis conjugated with thimerosal N-hydroxysuccinimide ester

Application

ELISA, WB

Species reactivity (positive)

Mycobacterium tuberculosis

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Clone number

11 G4

Epitope

Binds to residues 3-15 (EQQWNFAGIEAAA) of ESAT-6 (1)

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

ESAT-6 (6-kDa early secretory antigenic target) from M. tuberculosis  H37Rv (ATCC 27294) (1) is a secreted tubercular protein that forms a dominant target for cell-mediated immunity in the early phase of infection. The deduced amino acid sequence indicates a molecular mass of 10 kDa. It is present in M. tuberculosis, virulent strains of M. bovis, and in M. flavescens, M. kansasii, M. marinum,and M. szulgai, but not in avirulent M. bovis BCG substrains (3).