货号: | AAT-22251 |
供应商: | 艾美捷科技 |
英文名: | CytoTell Blue |
规格: | 500 Tests |
【产品形式】:艾美捷为您提供的蓝色活细胞荧光探针CytoTell Blue产品为固体形式,我们建议将其溶解到DMSO溶液中使用或保存。
【背景资料】:Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations. Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis. However, it is impossible to use CFSE and its fluorescein analogs for GFP-transfected cells or for the applications where a FITC-labeled antibody is used since CFSE and its fluorescein analogs have the excitation and emission spectra almost identical to GFP or FITC. CytoTell dyes are well excited at major laser lines such as 405 nm, 488 nm or 633 nm with multicolor emissions. CytoTell dyes have minimal cytotoxicity, and are used for the multicolor applications with either GFP cell lines or FITC-labeled antibodies since they have either excitation or emission spectra distinct from fluorescein. CytoTell Blue is a blue fluorescent dye that stains cells evenly. As cells divide, the dye is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye. Cells labeled with Cell CytoTell Blue may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers. CytoTell Blue has a peak excitation of 405 nm and can be excited by the violet (405 nm) laser line. It has a peak emission of 450 nm and can be detected with a 450/20 band pass filter (equivalent to Pacific Blue, or BD Horizon V450), making it compatible with applications that utilize GFP or FITC antibodies for multicolor cell analysis.
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括显色、荧光和生物发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学,免疫学,细胞生物学和分子生物学等领域。作为AAT Bioquest Inc.的中国区域代理,艾美捷科技为中国客户提供光谱学检测技术并应用于生化、生理代谢和细胞分析领域的产品,包括显色,荧光和发光技术等全系列解决方案。
点击蓝色活细胞荧光探针CytoTell Blue查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-868垂询AAT Bioquest中国区域授权总代理艾美捷科技有限公司。