货号: | ILA-H4213 |
供应商: | ACROBiosystems |
数量: | In Stock |
英文名: | ActiveMax® Recombinant Human IL-1 alpha / IL-1F1 |
规格: | 50ug |
Less than 1.0 EU per μg by the LAL method.
纯度:
>97% as determined by SDS-PAGE.
活性:
(1) Measured by its binding ability in a functional ELISA.Immobilized Human IL-1 RII, Fc Tag (Cat# IL2-H4256) at 10 μg/mL (100 µL/well),can bind ActiveMax® Human IL-1 alpha (Cat# ILA-H4213).The EC50 is typically 0.5-5 μg/mL.(2) The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells.The ED50 < 1pg/mL, corresponding to a specific activity of >1,000,000,000 Unit/mg.
产品背景:
Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA,IL1F1,and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum.The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, Shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes.
产品描述:
ActiveMax® Human IL-1 alpha contains no "tag", and has a calculated MW of 18 kDa. The reducing (R) protein migrates as 19-22 kDa in SDS-PAGE due to Glycosylation.