货号: | PDA-H4218-50ug |
供应商: | ACROBiosystems |
英文名: | Recombinant Human PDGF-AA Protein |
保存条件: | Lyophilized Protein should be stored at -5℃ or lower for long term storage. |
规格: | 50ug |
Recombinant human Platelet-derived growth factor AA ( rhPDGF-AA) Ser 87 – Thr211(Accession # P04085) was expressed in human 293 cells (HEK293) at ACRObiosystems.
MOLECULAR CHARACTERIZATION
rh PVRL1 /Nectin-1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 34.8 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation.
BIOLOGICAL ACTIVITY
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 4-10 ng/mL.
PURITY
>95% as determined by SDS-PAGE.
ENDOTOXIN
Less than 1.0 EU per μg of the rh PDGF-AA by the LAL method.