货号: | IL0-M4216-1mg |
供应商: | ACROBiosystems |
英文名: | Recombinant Mouse IL10 Protein,HEK293 expressed,ActiveMax |
保存条件: | Lyophilized Protein should be stored at -5℃ or lower for long term storage. |
规格: | 1mg |
Recombinant Mouse IL10 Protein,HEK293 expressed,ActiveMax (Mouse IL-10) Ser 19 - Ser 178 (Accession # NP_034678) was produced in human 293 cells (HEK293) at ACROBiosystems.
MOLECULAR CHARACTERIZATION
rh IL3 contains no "tag", and has a calculated MW of 15 kDa. The predicted N-terminus is Ala 20. DTT-reduced Protein migrates as 35-38 kDa monomer in SDS-PAGE due to glycosylation.
BIOLOGICAL ACTIVITY
The bio-activity was determined by dose-dependent co-stimulation (with IL-4) of the proliferation of mouse MC/9 cells. The ED50 was < 2 ng/mL, corresponding to a specific activity of > 5 x 105 units/mg.
PURITY
>95% as determined by SDS-PAGE.
ENDOTOXIN
Less than 1.0 EU per μg of the Mouse IL-10 by the LAL method.