Biotinylated Human B7-H4 Protein (recommended for biopanning), Avi Tag (Avitag™)

货号：	1211321315
克隆性：	否

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:

Immobilized Anti-B7-H4 mAb,Human IgG1 at 2μg/mL (100 μL/well) can bind Biotinylated Human B7-H4, His Tag (Catalog # B74-H82E2) with a linear range of 0.04-1.25 ng/mL.

SDS-PAGE:

Biotinylated Human B7-H4 (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

产品背景:
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.

产品描述:
Mouse Dkk-3, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 37.1 kDa. The predicted N-terminus is Pro 23. The reducing (R) protein migrates as 55-66 kDa in SDS-PAGE due to glycosylation.