β2-Glycoprotein 1 (Apo H; non recombinant; human)

货号：	11300
规格：	0.1mg

  Description: Human β2-glycoprotein 1 (β2-GP 1), also known as Apolipoprotein H or Apo H. Natural antigen for research use or manufacturing only.
  Immunological function: Binds IgG, IgA and IgM-type human auto-antibodies. Auto-antibodies to β2-glycoprotein 1 recognize conformation-dependent and linear epitopes.
  Origin: Native. Purified from human serum by protein-chemical methods.
  Biochemical tests: SDS-PAGE (purity > 90%); Western blot with polyclonal rabbit anti-ApoH antibody.
  Observed molecular weight (SDS-PAGE): Approx. 62 kDa
  Immunological tests/Functionality: Standard ELISA test (checkerboard analysis of positive/negative sera panels).
  Recommended buffer/storage and handling conditions: Recommendations for storage buffer: ionic strength around 250 mM, neutral to slightly alkaline pH and 20% glycerol as cryoprotective agent. Storage conditions: -70°C or below. Repeated freeze/thaw cycles should be avoided. Use of reducing sulfhydryl reagents and denaturing detergents will result in the destruction of conformational epitopes.
  Coating concentration: 0.5-1.0 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups. Due to the diversity of existing β2-glycoprotein 1/ApoH coating protocols (e.g. binding to phospholipid-precoated plates) further recommendations cannot be given.