货号: | 31466 |
抗体名: | 羊抗兔抗体 |
抗体英文名: | Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate |
浓度: | 1 mg/ml |
宿主: | 羊 |
标记物: | HRP |
克隆性: | 否 |
保存条件: | 4° C |
形态: | 液体 |
亚型: | IgG |
规格: | 0.2ml |
本抗体为库存产品,出场时间较长,数量有限,赠完为止。希望客户根据实验酌情选择。免费赠送产品无售后服务。需买家承担邮费。
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate
Rabbit IgG (H+L) Polyclonal Secondary Antibody for Western Blot, IF, ICC, IHC, Flow, IPDetails | |
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Host / Isotype | Goat / IgG |
Class | Polyclonal |
Type | Secondary Antibody |
Tested species reactivity | Rabbit |
Conjugate | HRP |
Target Class | IgG |
Tested Applications | Dilution * |
---|---|
Flow Cytometry (Flow) | 1:500 - 1:5,000 |
Immunocytochemistry (ICC) | 1:5,000 - 1:100,000 |
Immunofluorescence (IF) | 1:5,000 - 1:100,000 |
Immunohistochemistry (IHC) | 1:5,000 - 1:100,000 |
Immunoprecipitation (IP) | 1:500 - 1:5,000 |
Western Blot (WB) | 1:5,000 - 1:200,000 |
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Catalog#: 31466 Invitrogen™
Form information | |
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Form | Lyophilized |
Concentration | 1 mg/ml |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7.6, with 15mg/ml BSA, 50mM sucrose, proprietary stabilizer |
Preservative | no preservative |
Storage Conditions | 4° C |
Product specific information
Product # 31466 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31466 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species and with SuperBlock® Blocking Buffers.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 0.2 ml of distilled water (1 mg/ml after restoration).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene/Target Information
Thermo Scientific Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.