点击图片查看原图

17 Beta-ESTRADIOL β17唾液雌二醇

产品价格: ¥1.00

最小起订量:暂无 可售数量:暂无

发货时限:
暂无
所在地区:
中国北京
有效期至:
长期有效
最后更新:
2020-10-27 02:00:01
浏览次数:
16
企业信息

产品详情

品牌名称:
GENWAY
货号: GWB-7C30A3
Product Name: 17 β –Estradiol Saliva

# of Samples: 1 x 96 Assays

Intended Use: Competitive immunoenzymatic colorimetric method for quantitative determination of 17 β -Estradiol in saliva.

Introduction: Estradiol (17β-estradiol) is a sex hormone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including bone structure.
During the reproductive years most estradiol in women is produced by the ovaries, smaller amounts of estradiol are also produced by the adrenal cortex. In men, the testes produce estradiol. During pregnancy estrogen levels including estradiol rise steadily towards term. Estradiol increases due to placental production. In adult premenopausal women, ovarian estradiol production is stimulated by the interactions of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) throughout the menstrual cycle. In adult women, estradiol levels are measured in the evaluation of fertility and menstrual irregularities, and to monitor ovarian follicular function during induction of ovulation. In the female, estradiol acts as a growth hormone for tissue of the reproductive organs. The development of secondary sexual characteristics in women is driven by estradiol. Estradiol is involved in man fertility. Estradiol regulates the bone maintenance. Women who past the menopause experience an accelerated loss of bone mass due to a relative estrogen deficiency.
Estradiol affects the production of multiple proteins including lipoproteins, binding proteins, and proteins responsible for blood clotting. Estrogens have been found to have neuroprotective function.
Estrogen is considered an oncogene as it supports certain cancers, notably breast cancer and cancer of the uterine lining. In addition there are several benign gynecologic conditions that are dependent on estrogen such as endometriosis, leiomyomata uteri, and uterine bleeding.

Principles of the assay: Microtiter strip wells are precoated with anti-Estradiol antibodies (solid-phase). Estradiol in the sample competes with added horseradish peroxidase labelled Estradiol (enzyme-labelled antigen) for antibody binding. After incubation a bound/free separation is performed by solid-phase washing. The immune complex formed by enzyme-labelled antigen is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is inversely proportional to the amount of Estradiol in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorption at 450 nm is read using an ELISA microwell plate reader.

Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the dark.

Limitations of the Test: Sample(s), which are contaminated microbiologically, should not be used in the assay. Highly lipemic or haemolysed specimen(s) should similarly not be used. It is important that the time of reaction in each well is held constant for reproducible results. Pipetting of samples should not extend beyond ten minutes to avoid assay drift. If more than one plate is used, it is recommended to repeat the dose response curve. Addition of the substrate solution initiates a kinetic reaction, which is terminated by the addition of the stop solution. Therefore, the addition of the substrate and the stopping solution should be added in the same sequence to eliminate any time deviation during reaction. Plate readers measure vertically. Do not touch the bottom of the wells. Failure to remove adhering solution adequately in the aspiration or decantation wash step(s) may result in poor replication and spurious results.

References Joshi, U.M. et al. (1979) Steroids 34(1), 35Exley, D.and Abuknesha, R. (1978) Febs Letters 91(2), 162Ismail, A.A., Niswender, G.D. and Midgley, A.R. (1972) J. Clin:Endocrin. Metab 34, 177-184Rajkowski,K.M.,Cittanova N., Desfosses, B. and Jayle,M.F. (1977) Steroids 29 (5), 701-713Sadem, D., Sela, E. and Hexter, C.S. (1979) J. of Immunological Methods 28, 125-131Wisdom, G.B. (1976) Clin Chem. 22 (8), 1243-1255Worthmann, C.M., Stalling, J.F. and Hofmann, L.H. (1990) Clin. Chem. 36 (10), 1769-1773Lu, Y et al. (1999) Fertility and Sterility 71 (5), 863-868
温馨提示:不可用于临床治疗。

免责声明

本网页所展示的有关【17 Beta-ESTRADIOL β17唾液雌二醇】的信息/图片/参数等由的会员【北京盛科博源生物科技有限公司 】提供,由【生物实验采购网】会员【北京盛科博源生物科技有限公司 】自行对信息/图片/参数等的真实性、准确性和合法性负责,本平台(本网站)仅提供展示服务,请谨慎交易,因交易而产生的法 律关系及法律纠纷由您自行协商解决,本平台(本网站)对此不承担任何责任。您在本网页可以浏览【17 Beta-ESTRADIOL β17唾液雌二醇】有关的信息/图片/价格等及提供 【17 Beta-ESTRADIOL β17唾液雌二醇】的商家公司简介、联系方式等信息。

在您的合法权益受到侵害时,请您致电,我们将竭诚为您服务,感谢您对【生物实验采购网】的关注与支持!

网站首页 | 关于我们 | 联系方式 | 使用协议 | 版权隐私 | 网站地图  |  排名推广  |  广告服务  |  积分换礼  |  网站留言  |  RSS订阅  |  违规举报
 
免责声明:本站有部分内容来自互联网,如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权,请来电或致函告之,本网站将在规定时间内给予删除等相关处理。