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人enpp-1亲和纯化的多克隆抗体
R&D蛋白、抗体、试剂盒
2×蛋白质电泳loading buffer
[VIP第1年] 指数:1
1,2,4-三氯苯
Wako MagCapture™ Ex
30w/v% Albumin D-PBS
牛血清白蛋白,不含脂
HULK海藻酸降解酶 Hu
SuperSep Phos-tag™| 货号： | 291-58601 |
| 保存条件： | 冷藏 |
| 供应商： | Wako（和光纯药工业株式会社） |
| 英文名： | LabAssay ALP |
| 规格： | 900tests |
Alkaline Phosphatase (ALP) is distributed in a variety of tissues such as liver, bone, and small intestine in animals. The change of the enzyme activity in tissues is an im-portant hallmark for physiological phenomena as osteo-genesis and so on. This kit is for Alkaline Phosphatase assay in a simultaneous multi-sample assay format with a microplate using p-Nitrophenylphosphate as a substrate.
[Kit Contents]
- Substrate Tablet 20 tablets
(p-Nitrophenylphosphate Disodium 6.7 mmol/L, after dissolving) - Buffer Solution 100 mL
(2.0 mmol/L MgCl2, 0.1mol/L Carbonate Buffer, pH 9.8) - Stop Solution 100 mL(0.2 mol/L Sodium Hydroxide Solution)
- Standard Solution 10 mL
(0.5 mmol/L p-Nitrophenol Solution)
[Assay principle]
p-Nitrophenylphosphate is hydrolyzed into p-Nitrophenol and Phosphoric Acid in the carbonate buffer (pH 9.8) in the presence of Alkaline Phosphatase in sample. Released p-Nitrophenol showing yellow color is optically measured at 405 nm wavelength as the enzyme activity.
[Features]
Dynamic assay range: > 0.06 mmol/L
Standard assay range: 0 ~ 0.5 mmol/L (The activity at concentration more than 0.5 mmol/L can be determined by extrapolation due to its linearity.)
Reproducibility: C.V. < 10%
[Reference]
Yamamoto, M., Takahashi, Y., Tabata, Y.: Biomaterials, 24 (24), 4375 (2003).
