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磷脂酶D(PLD)活性检测试剂盒(比色法)

产品价格: ¥4425.00

最小起订量:暂无 可售数量:暂无

发货时限:
暂无
所在地区:
中国北京
有效期至:
长期有效
最后更新:
2020-06-20 02:00:02
浏览次数:
6

产品详情

品牌名称:
biovision
货号: K725-100
保存条件: -20度
供应商: 北京博迈斯
英文名: Phospholipase D (PLD) Activity Colorimetric Assay Kit
规格: 100 assays

Assay Kit Name:
Phospholipase D (PLD) Activity Colorimetric Assay Kit

Kit Summary:
• Detection method - Absorbance (OD 570 nm)
• Species reactivity - N/A
• Applications - Measurement of PLD activity in various tissues/cells
- Analyzing intracellular signaling
- Screening of PLD inhibitors

Sample Type:
• Animal tissues: liver, brain, kidney, etc.
• Cell culture: Adherent or suspension cells

Features & Benefits:
• Simple, rapid & convenient
• The assay can detect phospholipase D (PLD) Activity less than 1 mU/ml in various sample types.

Kit Components:
• PLD Assay Buffer
• PLD Probe (in DMSO)
• PLD Substrate
• PLD Enzyme Mix (Lyophilized)
• Choline Standard (Lyophilized)
• PLD Positive Control (Lyophilized)

Description:
Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders. BioVision’s Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from Phosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

温馨提示:不可用于临床治疗。

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