货号: | HC2401 |
数量: | 大量 |
规格: | 3 mL |
Description:
TurboLigationTM Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature. The kit combines TurboLigaseTM, an ultra-pure recombinant T4 DNA Ligase, and 5X TurboLigation Buffer, an optimized quick ligation buffer. A 5 minute TurboLigation reaction offers the same efficiency as a traditional 16oC overnight ligation reaction. The reaction product is ready for bacterial transformation without any further treatment.Applications:
- Adding linkers or adapters to DNA
- Blunt or cohesive end ligation of duplex DNA
- DNA cloning
Components:
TurboLigaseTM: 5 unit/µl in Storage Buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, and 50% glycerol5X TurboLigation Buffer: 250 mM Tris-HCl, pH 7.5, 50 mM MgCl2, 5 mM dithiothreitol, 5 mM ATP, 25% polyethylene glycol 8000
Stability and Storage:
TurboLigase and 5X TurboLigation Buffer are stable at -20oC for 12 months from date of receipt.Store TurboLigase and 5X TurboLigation Buffer at -20oC.
Shipped in gel packs.
DATA SHEET
TurboLigaseTM Unit Definition:
0.015 Weiss unit (1 cohesive end ligation unit) is defined as the amount of TurboLigaseTM required to give 50% ligation of HindIII fragments of l DNA (5´ DNA termini concentration of 0.15 µM) in a total reaction volume of 20 µl in 30 minutes at 16°C in a buffer of 50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mM ATP, 10 mM dithiothreitol.TurboLigaseTM Specific Activity and Purity:
TurboLigase has a specific activity >12,000 unit/mg.TurboLigase is purified to homogeneity.