货号: | 10006748 |
供应商: | 广州威佳科技有限公司 |
数量: | 大量 |
规格: | 96 wells 、480 wells |
Limit of detection: 80% B/B0: 18 pg/ml · Sensitivity: 50% B/B0: 66 pg/ml · The leukotrienes (LTs) were discovered in 1979 as a group of acute inflammatory mediators derived from arachidonic acid in leukocytes. Although the majority of leukotrienes are formed through the 5-lipoxygenase (5-LO) pathway, a second family of leukotrienes can be formed through an alternate pathway involving the dual actions of 15- and 12-LOs.1,2,3,4 The resulting epoxytriene, 14,15-LTA4, can be converted to 14,15-LTC4, as demonstrated in sheep uterus and RBL-1 cells. Although many of the physiological actions of 14,15-LTC4 remain to be elucidated, it has been shown to have weak contractile activity on both guinea pig ileum and pulmonary parenchyma.5 |
1 Yokoyama, C., Shinjo, F., Yoshimoto, T., et al. Arachidonate 12-lipoxygenase purified from porcine leukocytes by immunoaffinity chromatography and its reactivity with hydroperoxyeicosatetraenoic acids. J Biol Chem 261 16714-16721 (1986). 2 Bryant, R.W., Schewe, T., Rapoport, S.M., et al. Leukotriene formation by a purified reticulocyte lipoxygenase enzyme. Conversion of arachidonic acid and 15-hydroperoxyeicosatetraenoic acid to 14,15-leukotriene A4. J Biol Chem 260 3548-3555 (1985). 3 Sok, D., Han, C., Shieh, W., et al. Enzymatic formation of 14,15-leukotriene A and C(14)-sulfur-linked peptides. Biochem Biophys Res Commun 104(4) 1363-1370 (1982). 4 Sailesh, S., Kumar, Y.V.K., Prasad, M., et al. Sheep uterus dual lipoxygenase in the synthesis of 14,15-leukotrienes. Arch Biochem Biophys 315(2) 362-368 (1994). 5 Drazen, J.M., Lewis, R.A., Austen, K.F., et al. Contractile activities of structural analogs of leukotrienes C and D: Necessity of a hydrophobic region. Proc Natl Acad Sci USA 78(5) 3195-3198 (1987). |