The USDA grade certified Fetal Bovine Sera (FBS) qualified for Mesenchymal Stem Cells are high quality pre-screened FBS batches. It saves time and efforts by eliminating the need to test multiple lots for performance. mesenchymal stem cell qualified FBS are screened for the following: - Ability to differentiate
- Morphology
- Cell growth and expansion
- Clonal efficiency
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Each lot of FBS is extensively tested for its ability to support in vitro growth and expansion of undifferentiated hMSCs in two culture systems: |
I. Promotion of cell growth and expansion |
| hMSC-BM cultured in DMEM low-Glucose supplemented with 10% of Biological Industries' MSC Qualified FBS | |
II. Clonal efficiency |
Mesenchymal Stem Cell Colony Forming Unit-Fibroblast (CFU-F) assay was used to evaluate the self renewal potential of hMSC in tested conditions. Optimized serum will induce higher numbers ofMesenchymal Stem Cell colonies when cultured at very low seeding density. |
| Represented colony stained with 0.5% crystal violet following 14-day CFU-F assay of hMSC-AT cultured in DMEM low-glucose supplemented with 10% of Biological Industries' MSC Qualified FBS. | |