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Anti-Active Rap1 Mouse Monoclonal Antibody

产品价格: ¥4800.00

最小起订量:暂无 可售数量:暂无

发货时限:
暂无
所在地区:
中国湖北武汉市
有效期至:
长期有效
最后更新:
2021-05-30 01:30:16
浏览次数:
15

产品详情

品牌名称:
NewEastBio
货号: 26912
抗体名: Rap1
抗体英文名: Rap1-GTP antibody
靶点: Rap1
浓度: 1mg/ml
应用范围: IP、IHC
宿主: Mouse
供应商: NewEastBio
数量: 30000
级别: 实验级
目录编号: 26912
抗原来源: Mouse
保质期: 两年
适应物种: 脊椎动物
标记物: FITC
克隆性:
保存条件: -20℃
形态: 液体
亚型: IgG
免疫原: Mouse
规格: 30μl
Active Rap1-GTP [26912]
[30 µL]
 
 
Catalog Number:  26912
Gene Symbol:  RAP1
Description:   Anti-Active Rap1 Mouse Monoclonal Antibody
Background:   Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins.
Immunogen:   Recombinant full length protein of active Rap1.
Applications:   IP, IHC
Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP
1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Rap1 antibody recognizes active Rap1 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western blot:  
Rap1 activation assay. Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rap1 monoclonal antibody (Cat. No. 26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. 21104).
Publications:
1.  Rap1 GTPase Activation and Barrier Enhancement in RPE Inhibits Choroidal Neovascularization In Vivo
    PLoS One. 2013 Sep 10;8(9):e73070
温馨提示:不可用于临床治疗。

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