DMEM/F-12(1:1)培养基

货号：	C11330500BT
CAS号：	详询
供应商：	弗尔博生物
数量：	500ML
保存条件：	2-8°避光
规格：	500ML

产品名称：DMEM/F-12(1:1)培养基
产品型号：C11330500BT
产品用途：用于科学研究及细胞体外培养，不用于临床诊断。                                                                                  
描述
Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

This F-12K (Kaighn's) Medium is manufactured as follows:
With Without
• L-glutamine • HEPES
• Phenol Red 

The complete formulation is available.

Using Ham's F-12K
Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture Ham's F-12K (Kaighn's) Medium at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.