点击图片查看原图
HGC-27(HGC27)；人胃癌细胞（未分化）
AC16
大鼠脑膜细胞 RMC
通过南京市浦口区市场监督管理局认证 
| 细胞类型： | 其他细胞类型 |
| 运输方式： | 冻存运输 |
| 是否是肿瘤细胞： | 1 |
| 物种来源： | 人 |
| ATCC Number： | CRL-8024™ |
| 相关疾病： | 肝癌 |
| 数量： | 大量 |
| 器官来源： | 肝 |
| 生长状态： | 贴壁生长 |
| 细胞形态： | 上皮样 |
| Designations: | PLC/PRF/5 | ||
| Depositors: | WJ McAleer | ||
| Biosafety Level: | 2 [Cells Contain HEPATITIS B ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial | ||
| Source: | Organ: liver Disease: hepatoma Cell Type: Alexander cells; | ||
| Cellular Products: | hepatitis virus B surface antigen (HBsAg) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10 D13S317: 11,12 D16S539: 13 D5S818: 12 D7S820: 9,11 THO1: 8 TPOX: 8 vWA: 15,16 | ||
| Comments: | The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline. The cells secrete HBsAg. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C | ||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. | ||
| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 | ||
| References: | 22189: Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321 22198: MacNab GM, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208 22236: Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138 22244: . . Science 209: 407, 1980. 22265: . . S. Afr. J. Med. Sci. 41: 8-9, 1976. 23123: Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281 23525: Alexander JJ, et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998 | ||
