货号: | M54010010 |
HIT-Blue--Xl-Blue 感受态细胞
货号: | M54010100 |
规格: | HIT-Blue: 100 µL×10 Control plasmid: 10 µL×1 |
价格: | ¥320.00 |
储存: | -70°C储存至少6个月转化效率不变 |
操作演示: | HIT 感受态细胞转化操作视频 |
ac[F'proAB acIqZDM15Tn10(Tet )]
- 无需热刺激
- 10min以内完成转化步骤
- 真正1步/1管过程
- 效率高达 108 cfu/ug 质粒
- 增加冻融耐性
- 简单的操作过程适合于自动化
(1-10 minutes, efficiency=108 cfu/µg)
注意:转化前请准备好冰浴,将平板培养基与涂布玻璃珠干燥预热到37℃
- 1) 从-70℃冰箱取出感受态细胞置于冰浴中10~20秒,至解冻1/3。
- 2) 加入待转化的DNA(其中DNA 体积不应超过感受态细胞体积的1/10)。漩涡振荡离心管1秒,混匀内容物。
- 3) 控制冰浴中静置1-10分钟(建议可以静置5分钟)。
- 4) 转移至37℃干燥平板培养基(含相应抗生素的SOC或LB固体琼脂培养基等)上, 用ViVaCell涂布玻璃珠涂板。
- 5)平板置于 37℃培养16-18 小时,观察转化克隆的增长
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