过氧化氢酶

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Biochem/physiol Actions

激活过氧化氢的分解，用于研究活性氧在基因表达和细胞凋亡中的作用的天然抗氧化剂。

This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Caution

不建议冷冻溶液。

包装

1, 5, 10, 50 g in poly bottle

Preparation Note

由缩二脲测定的蛋白质

Unit Definition

在 pH 7.0 和 25°C 下，一单位本品每分钟可以分解 1.0μmol H₂O₂，同时 H₂O₂ 浓度从 10.3mM 降至 9.2mM，这是通过 A₂₄₀ 的降低速率来测量的。