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Human Protein C ELISA Kit (人蛋白C酶联免疫试剂盒)

产品价格: ¥4760.00

最小起订量:暂无 可售数量:暂无

发货时限:
暂无
所在地区:
中国上海
有效期至:
长期有效
最后更新:
2021-05-30 01:30:16
浏览次数:
17

产品详情

品牌名称:
AssayMax
货号: EP1311-1
样本: 25 μl
应用: 血清、血浆
检测方法: ELISA
检测限: 0.09 μg/ml
供应商: AssayPro
数量: 大量
规格: 96 wells
AssayMax Human Protein C ELISA Kit
人蛋白C酶联免疫试剂盒

 
Introduction
Protein C is a vitamin K-dependent plasma antithrombotic and anti-inflammatory zymogenic glycoprotein that is synthesized in the liver. Protein C has a light chain of 155 amino acids (21 kDa) and a heavy chain of 262 amino acids (41 kDa) linked by a disulfide bond. On endothelial cell membrane, thrombin-thrombomodulin complex cleaves a 12-residue peptide from protein C amino terminus of the heavy chain and converts it to activated protein C (APC). APC inactivates coagulation Factor Va and Factor VIIIa and performs a major role in regulating blood clotting, inflammation, and apoptosis (1 - 3). Protein C deficiency causes neonatal purpura fulminans, thrombophilia, and recurrent venous thrombosis (4 - 6). Protein C pathway components have been studied in the treatment of complex disorders, including severe sepsis, thrombosis, and ischemic stroke (7).
 
Principle of the Assay
The AssayMax Human Protein C ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human Protein C in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human protein C in less than 3 hours. A polyclonal antibody specific for human protein C has been pre-coated onto a 96-well microplate with removable strips. Protein C in standards and samples is competed with a biotinylated protein C sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
 
温馨提示:不可用于临床治疗。

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