Human Complement C3 ELISA Kit（补体C3酶联免疫试剂盒）

货号：	EC3201-1
样本：	50 μl
应用：	细胞培养上清液、尿液、唾液、牛奶
检测方法：	ELISA
检测限：	0.6 ng/ml
供应商：	AssayPro
数量：	大量
规格：	96 wells

Introduction
Complement component 3 (C3) plays a central role in all three complement activation pathways. The C3 precursor contains 1663 amino acids and has a molecular weight of about 180 kDa (1). Human C3 has 77% identity to mouse C3 at the amino acid level (2). C3 is cleaved by C3 convertase into two activated fragments C3a and C3b. The anaphylatoxin C3a is a vasoactive peptide and a mediator of local inflammatory process (3). The C3b in complex with receptor can bind covalently to pathogen surfaces to promote phagocytosis (4-5). Acquired C3 deficiency is associated with severe recurrent meningococci and pneumococci infections (6). Plasma C3 and C3a levels are elevated in cryptogenic and large-vessel disease subtypes of ischemic stroke (7).
 
Principal of the Assay
The AssayMax Human Complement C3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human complement C3 in urine, milk, saliva and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human complement C3 in less than 4 hours. A polyclonal antibody specific for human complement C3 has been pre-coated onto a 96-well microplate with removable strips. Complement C3 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Complement C3, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.