AssayPro—Mouse alpha-Microglobulin ELISA Kit（小鼠α-微球蛋白α-MG酶联免疫试剂盒）

货号：	EMM1115-1
样本：	50 μl
应用：	血清、血浆、细胞培养上清液、尿液
检测方法：	ELISA
检测限：	0.07 ng/ml
供应商：	AssayPro
数量：	大量
规格：	96 wells

Introduction
Alpha-2-Macroglobulin is a major serum protein with diverse functions, including inhibition of protease activity and binding of growth factors, cytokines, and disease factors (1). Increased serum α2-macroglobulin has been suggested to be associated with multiple sclerosis (MS) (2), glomerular disease (3), and liver diseases (4). Mouse α-macroglobulin (Mouse AMG) is believed to be a functional homologue of human α2-macroglobulin (Human α2M).
 
Principle of the Assay
The AssayMax Mouse α-Macroglobulin ELISA kit is designed for detection of mouse α-macroglobulin in mouse plasma, serum, urine, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures mouse α-Macroglobulin in less than 4 hours. A polyclonal antibody specific for α-Macroglobulin has been pre-coated onto a microplate. Alpha-macroglobulin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for α-macroglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.