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Methylcholanthrene Antibody (OADC00002)

产品价格: ¥2376.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国北京
有效期至:
长期有效
最后更新:
2021-04-11 01:30:02
浏览次数:
25

产品详情

品牌名称:
Aviva Systems Biology
货号: OADC00002
抗体英文名: Methylcholanthrene Antibody (OADC00002)
应用范围: DB
宿主: Rabbit
适应物种: Human
标记物: Unconjugated
克隆性:
保存条件: Store at -20C. Avoid repeated freeze/thaw cycles.
形态: Liquid. Provided in PBS containing 0.05% azide and 0.05% ProClin 300.
规格: 50 ug
3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OADC00002

Methylcholanthrene Antibody (OADC00002) in 3-mCr using Dot Blot

3-mCr

Dot blot analysis using the Aviva antibody directed against 3-mCr. To demonstrate the specificity of the Aviva antibody against 3-mC, a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo's were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skim milk and 1% BSA. Figure shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.

温馨提示:不可用于临床治疗。

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