EA.hy926，huvec与A549融合株

产品价格: ￥600.00

最小起订量：暂无可售数量：999盒

发货时限：: 暂无

所在地区：: 中国上海

有效期至：: 长期有效

最后更新：: 2021-05-30 01:30:16

浏览次数：: 24

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货号：	C0968
生长状态：	贴壁
运输方式：	冻存或复苏
ATCC Number：	CRL-2922™
供应商：	上海觅拓生物
规格：	T25

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Organism	Homo sapiens, human
Tissue	somatic cell hybrid
Cell Type	endothelial
Product Format	frozen
Morphology	endothelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation.
Storage Conditions	liquid nitrogen vapor phase

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 103 to 3 X 103 viable cells/sq. cm is recommended. Incubate cultures at 37°C. Subculture when cell concentration reaches between 8 X 104 and 1 X 105 cells/sq. cm. Interval: Twice a week Subcultivation Ratio: A seeding density of 2 X 103 to 3 X 103viable cells/sq. cm should be used when subculturing these cells Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Complete Growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C

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