货号: | R1010 |
供应商: | 无锡柏欧美地生物 |
保质期: | 24个月 |
数量: | 大量 |
保存条件: | 室温 |
规格: | 50ml,100ml |
RNAtrip总RNA提取试剂 | ||||||||
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价格说明:市场的9折,量多优惠,欢迎洽谈! 描述: RNAtrip提取试剂成功避开各种商业RNA提取试剂盒的种种弊端,采用最新技术和工艺,在提取过程中既能有效裂解组织细胞,强烈抑制RNA酶活性保护RNA免受降解;同时极为有效地分离去除DNA和蛋白质,获得极高纯度的总RNA沉淀。可用于RT-PCR、Northern Blot、RNA酶保护、Poly(A) mRNA纯化、体外翻译等实验。RNAtrip使RNA分离如同提取质粒DNA一样简单可靠,仿佛进行一次轻松的旅行。 抑制RNA酶更强烈 去除DNA和蛋白质更彻底 RNA纯度极高 一步法,30分钟提取 实验前准备更简单 适用: (1) 组织 (100 mg/ml ): 新鲜或冻存的人、动物、植物组织。 (2) 细胞 (5-10 x 106/ml): 真核细胞,细菌,酵母。 (3) 液体 (100 µl/ml): 全血、体液、尿液,病毒样品等。 左下图:RNAtrip试剂提取大鼠几种组织和小鼠原代心肌细胞总RNA1%常规琼脂糖凝胶。Lane 1, 大鼠骨骼肌;Lane 2,肝脏;Lane 3,心脏;Lane 4,小鼠原代心肌细胞。用RNAtrip提取的28S与18S RNA的亮度比例在接近3:1,明显超出通常的2:1,而凝胶前沿5S RNA处几乎没有降解RNA;同时加样孔和泳道未见高亮度的基因组DNA的污染残留,表明RNAtrip提取的RNA纯度和完整极高、没有降解。 右上图:RNAtrip试剂提取棉铃虫胸部组织总RNALane 1-3上样量递增。棉铃虫18S RNA的含量大于28S RNA。RNAtrip提取的高纯度棉铃虫胸部组织总RNA,18S RNA最亮,5S RNA处几无降解RNA,加样孔无高亮度的基因组DNA污染残留。 保存: 4ºC避光储存1年。25ºC室温避光存放数月质量不受影响。 部分发表论文: Zhu Minjia, Fu Yi, Hou Yingjian, Wang Nanping, Guan Youfei, Tang Chaoshu, Shyy John Y.-J., Zhu Yi, 2008. Laminar Shear Stress Regulates Liver X Receptor in Vascular Endothelial Cells. Arteriosclerosis, Thrombosis, and Vascular Biology, 28: 527-533. IF: 7.215 Yang Guangrui, Chen Lihong, Zhang Yahua, Zhang Xiaoyan, Wu Jing, Li Shuo, Wei Mingfen, Zhang Zhiwen, Breyer Matthew D., Guan Youfei, 2006. Expression of mouse membrane-associated prostaglandin E2 synthase-2 (mPGES-2) along the urogenital tract. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1761(12): 1459-1468. IF: 5.084 Xu Chong, He Jinhan, Jiang Hongfeng, Zu Luxia, Zhai Wenjie, Pu Shenshen, Xu Guoheng, 2009. Direct Effect of Glucocorticoids on Lipolysis in Adipocytes. Molecular Endocrinology, 23(8): 1161-1170. IF: 4.889 Zhang Yongjie, Liu Xingzhong, Wang Mu, 2008. Cloning, expression, and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic fungus Cordyceps sinensis. Research in Microbiology, 159(6): 462-469. IF: 2.405 Zhang Dong-juan, Chen Li-hong, Zhang Ya-hua, Yang Guang-rui, Dou Dou, Gao Yuan-sheng, Zhang Xiao-yan, Kong Xiao-mu, Zhao Pan, Pu Dan, Wei Ming-fen, Breyer Matthew-D, Guan You-fei, 2010. Enhanced pressor response to acute Ang II infusion in mice lacking membrane-associated prostaglandin E2 synthase-1. Acta Pharmacologica Sinica, 31: 1284-1292. IF: 1.909 |