点击图片查看原图
DH5a
pET-21b
(R)-(+)-2,2'-二(二-3,5-甲基苯
通过天津市南开区市场和质量监督管理局认证 
PBS磷酸盐缓冲液 2L s
泡沫浮漂 水浴锅浮漂1
RayBio Legionella Se
一次性针式滤器 密理
RayBio Kremen-1 ELIS
天津 现货 DMSO 二甲| 品牌： | Invitrogen |
| 货号： | V800-20 |
| 数量： | 大量 |
| 供应商： | 天津子涵生物科技有限公司 |
| 规格： | 100 TESTS |
and designed for high-level expression, purification, and detection of
recombinant proteins in mammalian hosts. High-level stable and non-replicative
transient expression can be carried out in most mammalian cells. The vectors
contain the following elements:
Three reading frames to facilitate in frame cloning with a C-terminal tag
encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide
Human cytomegalovirus immediate-early (CMV) promoter for high-level
expression in a wide range of mammalian cells
Episomal replication in cell lines that are latently infected with SV40 or that
express the SV40 large T antigen (e.g., COS7)
The control plasmid, pcDNA™3.1/myc-His/lacZ, is the pcDNA™3.1/myc-His C
vector with a 3.2-kb fragment containing the -galactosidase gene cloned in frame
with the C-terminal peptide (see page 9). It is included for use as a positive
control for transfection, expression, purification, and detection in the cell line of
choice.
