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大肠杆菌Origami B（DE3)菌种
Grams iodine solution
Alexa Fluor 350标记的兔抗猪IgG
蓝盖试剂瓶
0.2mlPCR管Taq Enzyme
PCR Reaction Kit Components:
(reagents are sufficient for 200 reactions of 50ul volume)
Taq enzyme(5units/ul) 50uL
dNTP(2.5mM of dATP dCTP dGTP and dTTP) 800uL
10Xreaction buffer (15mM MgCI2) 1mL
Applications
1.polymerase chain reaction to amplify DNA
2. Sequencing
Typical PCR Reaction Mixture:
(for a 50uL reaction)
Taq enzyme(5unit/uL) 0.25uL
Template DNA <200ng
10Xbuffer 5uL
dNTP(2.5mM of each) 4uL
Primers 10-50pmol of each
Nuclease-free water to 50uL
