货号: | ICP9807 |
抗体名: | Phospho-Threonine 抗体 |
抗体英文名: | Phospho-Threonine Antibody |
浓度: | 250 µg/mL |
克隆性: | 否 |
规格: | 100 µg |
Product Description | Affinity purified rabbit polyclonal phospho-threonine antibody (anti-pT) was developed using a phospho-threonine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-threonine specific antibody was affinity purified with phospho-threonine immobilized on agarose. | | Detection of a phosphorylated protein kinase with anti-phosphothreonine (ICP9807) Lane1-2: Untreated kinase Lane 3-4: Kinase treated with phosphatase PP1G Western blot analysis of mouse extract spleen with ICP9807 A: Extract of spleen from mouse treated with sodium vanadate B: Extract of spleen from control mouse |
Species | Rabbit | ||
Formulation | 250 µg/mL in PBS, pH 7, with 50% glycerol | ||
Immunogen | Phospho-threonine KLH conjugates and phosvitin mixture | ||
Purification | The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose. | ||
Specificity | The antibody recognizes proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phospho-tyrosine. | ||
Species Tested | Human and mouse | ||
Applications | ELISA; WB; IP | ||
Scientific Description | Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phospho-threonine which enters various biosynthetic pathways in the body. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
References | 1. M.J. Betts, R.B. Russell. Bioinformatics for Geneticists, M.R. Barnes, I.C. Gray eds, Wiley, 2003. |