Anti-14-3-3 delta + zeta (phospho T232)抗体

• 特异性ab195909 detects endogenous levels of 14-3-3 delta + zeta only when phosphorylated at Threonine 232.
• 经测试应用WBmore details
• 种属反应性
  与反应: Mouse, Rat, Human
• 免疫原

  Synthetic peptide corresponding to Human 14-3-3 delta + zeta (phospho T232). (Derived from around the phosphorylation site of Threonine 232). Database link: P63104

• 阳性对照
  • UV treated Jurkat whole cell lysate.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride
• 浓度100 µg 浓度为 1 mg/ml 
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG

应用

靶标

• 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• 序列相似性Belongs to the 14-3-3 family.
• 翻译后修饰The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.
• 细胞定位Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 7534 Human
  • SwissProt: P63104 Human
  • Unigene: 492407 Human
• 别名
  • 14-3-3 protein zeta/delta antibody
  • 1433Z_HUMAN antibody
  • KCIP-1 antibody

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Anti-14-3-3 delta + zeta (phospho T232) antibody 图像

• 

  Western blot - Anti-14-3-3 delta + zeta (phospho T232) antibody (ab195909)
  All lanes : Anti-14-3-3 delta + zeta (phospho T232) antibody (ab195909) at 1/500 dilution Lane 1 : UV treated Jurkat whole cell lysate with antigen specific peptide Lane 2 : UV treated Jurkat whole cell lysate Lysates/proteins at 12.5 µg per lane. Predicted band size : 28 kDa